In our phone meeting last week we briefly touched the issue of picking a secondary antibody to conjugate with QDs. Of course, the discussion actually started when we were in Georgia. Here is the original suggestion from Beth’s student Julia
<“Goat anti-mouse IgG2b” but we can change the goat part if necessary.>
I realized that my lab will be frequently using mouse hippocampal neurons from now on (although we will continue to use anti-mouse secondary for Drosophila). So for secondary antibody my lab may also need to choose “Goat anti-rabbit IgG”. But I understand that Beth’s lab will also be using single muscle cells from rabbit (if I recall correctly). In view of this, we might need to have two secondary antibody options instead of one. I understand that this would mean more work and expense for Jessica’s lab. Your thoughts will be much appreciated. I hope that we can arrive at a decision soon.
-Ge [via email]