Monthly Archives: June 2011

TEM looks promising…

Cally and Julia did some more TEM imaging this afternoon. This image is from part of the tail that did not have qdots injected, but it was difficult to get good images of that part of the sample. It does more »

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Re: Fish for the Kner lab – Julia

June 30, 2011: Hi Beth, We are using Alexa 488 right now to stain collagen. Do you want us to order more antibodies and switch (making the muscle Alexa 488 and the collagen Alexa 561)? -Julia

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Fish for the Kner lab – Beth

June 30, 2011: Hi Julia — please find out whether Alexa 488 is available as a label on our secondary antibodies. This would be best for imaging fish in Peter Kner’s lab. Also — can you tell how thick the more »

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TEM of muscle with qdots

Julia collected this TEM image of muscle with qdots microinjected. We do not know whether the black dots are qdots or not. The black dots may come from the stain. We will collect some control images of tissue without qdots more »

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Matching Laser to Label

Hi Peter — To answer your question from our phone conversation, the fluorophore label on our secondary antibody is an Alexa 568 dye. http://products.invitrogen.com/ivgn/product/A21144?ICID=search-a21144 We can use a different label if that would be better for the lasers that you more »

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Problem with Texas Red and qdots

Hi Ge — To follow up on what I said on the phone today, we tried mixing the micelle coated qdots with Texas Red dye, and could not image the qdots (but could image the Texas Red). When we imaged more »

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New quantum dot synthesis – first experiment

These quantum dots are supposed to be switchable (Mn-doped ZnSe quantum dot). Just got the first batch. Under room light the color of solution is yellow (not sure if the color is from fluorescence though). Will perform more purification and more »

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Immunohistochemistry of zebrafish myosin–Julia

Cally and I labeled zebrafish myosin with MF20 via whole mount immunohistochemistry. Then we imaged the fish with the confocal microscope. The first video is a 40x video of the fish muscle. The A-bands are bright, the I-bands are dark, more »

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